A simple enzyme-linked immunosorbent assay of human osteocalcin

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A simple enzyme-linked immunosorbent assay of human osteocalcin.

A heterologous ELISA for measurement of osteocalcin (bone Gla-protein) is described, involving biotinylated bovine osteocalcin and polyclonal antibodies. The log-linear range was 2.3-37.5 micrograms/L. Between-run (total) and within-run CVs (n = 10) were 5.7-6.4 and 5.9-6.1%, respectively; analytical recoveries ranged from 92% to 108%. Comparison of our method (x) with an RIA (y) yielded y = 1....

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1994

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/40.7.1258